Single step large scale site-directedin vitromutagenesis using multiple oligonucleotides
نویسندگان
چکیده
منابع مشابه
An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol
BACKGROUND Mutagenesis plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which are more tractable for biophysical techniques. The ability to quickly and specifically mutate a residue(s) in protein is important for mechanistic and functional studies. Although many site-directed mutagenesis methods have been...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.24.7457